Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
one-step purification of recombinant enzyme | Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
120 | - |
- |
Geobacillus stearothermophilus |
Storage Stability | Organism |
---|---|
4°C, buffer containing 0.02% sodium azide, stable for more than 1 year | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Leu + H2O + NAD+ | - |
Geobacillus stearothermophilus | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
pH 6.0-11.0, 10 min, stable | Geobacillus stearothermophilus |
70 | - |
30 min, retains full activity | Geobacillus stearothermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | 55°C, 10 min, stable | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Geobacillus stearothermophilus | |
NADH | - |
Geobacillus stearothermophilus |